Cant he One-Step Nucleic Acid Amplification method of lymph nodes examination make the staging of pulmonary tumours more precise?
Abstract
The aim of this article is to compare the sensitivity of detecting micrometastases in hilar and mediastinal lymph nodes in case of primary and secondary pulmonary tumours using standard histopathological examination with haematoxylin-eosin staining, immunohistochemistry examination with Anti-Cytokeratin 19 antibody and examination based on the OSNA method. The conclusion can be said that the examination of lymph nodes using the OSNA method can have a certain potential to make the pulmonary tumours staging more accurate.
On the other hand, imunohistochemistry with AntiCytokeratin 19 antibody seems to be not so useful. However, it is necessary to prove this hypothesis in follow-up studies, or where applicable, in a larger cohort of patients.
Another task is to ascertain, by careful patient monitoring, the influence of the micrometastases detected in their lymph nodes using the OSNA method on these patients' follow-up