Monitoring of adipose tissue metabolism using microdialysis and capillary electrophoresis with contactless conductivity detection
Abstract
Metabolism of human adipose tissue during acute exercise is monitored by microdialysis. The metabolites are analysed in the microdialysate using capillary electrophoresis (CE) on a short separation path in combination with a contactless conductivity detector (C4D).
Four completely new CE/C4D methods were developed for determination of nutrients and metabolites in 10 µL of microdialysate. All methods are characterised by a short separation time and simple sample preparation based primarily on 4-fold dilution of microdialysate.
The intra-day repeatability for the migration time varied in the range 0.4 - 0.9% and that for the peak area equalled 0.7 - 2.4%; the inter-day repeatability of the migration time was in the range 1.2 - 2.3% and the range for the peak area was 2.5 - 5.0%; all the values were measured as RSD. The developed determination was employed for sequenced monitoring of the levels of lactate, glycerol and branched chain amino acids in microdialysates taken from the abdominal adipose tissue during acute physical exercise.
The stress test lasted 3 h and the metabolites were monitored at 15 min intervals.