Effect of Precursor and Phytohormones on Podophyllotoxin Production in Juniperus virginiana Suspension Cultures
Abstract
Our results showed that cinnamic acid can increase podophyllotoxin production in Juniperus virginiana suspension cultures. The best effect was manifested after a 24-hours application of a 10 mmol/L concentration.
The highest podophyllotoxin content was determined at 1.47 mg/g DW and the production was statistically significantly stimulated by about 444% in comparison with the control. Comparison of podophyllotoxin production in the cinnamic acid- and salicylic acid-elicited J. virginiana suspension cultures confirms that the maximum increase in both cases was induced by the 24-hours application of the 10 mmol/L concentration.
In contrast, the best effect of jasmonic acid was manifested after the longest 168-hours application of a 5 mmol/L concentration.