Fluorescent proteins (FP) proved to be a useful tool for studying biological processes in living cells at the molecular level. For the first time, these proteins were isolated and studied more than half a century ago.
Over time, their functions, structure and properties have been elucidated, and many possibilities have been described for their use in biomedical research. The aim of this article is to make PMFA readers acquainted with basic knowledge about the structure and optical properties of this extremely interesting group of proteins, as well as the main fields of their practical applications.