First experiences with detection of the tcdB gene of Clostridioides difficile using Simplexa C.difficile Direct Kit
Abstract
Objective: Clostridioides difficile (formely Clostridioidis difficile) is one of the main pathogens causing nosocomial infections today. It colonizes the intestines of patients receiving antibiotic therapy, causing unpleasant or even life-threatening conditions(diarrhea, toxic megacolon).
Rapid and correct detection of strain toxigenicity is essential for treatment and isollation of patients. Simplexa C. difficile Direct Kit is a real-time PCR kit detecting the tcdB gene of C. difficile The kit does not require DNA isolation; stool eluates are directly used for the reaction.
The study aimed to verify the analytical properties of the kit used in the third diagnostic step (assessment of samplex yielding unclear ruesults). Culture and subsequent in -house multiplex PCR were used as a reference method.