Correlation of expression of Ki-67, MCM2, MCM5 and p53 in tissue specimens and cell blocks in patients with urothelial carcinoma
Abstract
Major statement: The study deals with immunocytochemistry as an additional method to urinary cytology examination. We present the correlation of selected immunochemical markers expression in tissues and cell blocks in patients with urinary bladder urothelial carcinoma.
Aim: The aim of the study is to verify whether expression of selected immunochemical markers in tumor tissue of patients with urinary bladder urothelial carcinoma correlates with expression in cytology (cell block) material. Material and methods: In 77 patients (25 with low-grade urothelial carcinoma, 52 with high-grade carcinoma) we performed the immunochemical examination of Ki-67, MCM2, MCM5 and p53 markers in tumor tissues and cell blocks from urine collected before surgical resection.
Thereafter we correlated the expression rate of each marker in tissue specimen and cell block in every patient. Results: There were significant differences in expression of all examined markers between low-grade and high-grade urothalial carcinomas in tissue specimens and cell blocks.
Expression of all markers in tissue specimens correlated with expression in cell blocks. Conclusion: Immunocytochemical examination of material retrieved from cell block with adequate cellularity has similar value as immunohistochemical examination of tumor tissue.