Confocal micro X-ray fluorescence spectroscopy (confocal micro-XRF) has recently become a useful instrumental method for analyses of art and archaeological objects. Depth-resolved information on elemental distribution can be obtained by scanning the sample perpendicularly to its surface (depth-profiling).
Thus, it is possible to distinguish single layers in multilayered samples in a non-destructive way. This work describes results of confocal micro-XRF analyses of paint layers of Bohemian panel paintings from the half of the 15th century that are part of the collections of the National Gallery in Prague.