Enzymatic kinetic resolution of chiral sulfoxides - an enantiocomplementary approach

Abstract

A new enzymatic assay for the preparation of chiral sulfoxides that is enantiocomplementary to the known (S)-enantiomer-reducing activity of methionine sulfoxide reductase A (MsrA) is described. To this end, we have utilized the enzyme DMSO reductase (DmsABC), recently discovered by us being highly upregulated in stationary phase E. coli bacteria.

Keywords

• directed evolution
• Escherichia-coli
• laboratory evolution
• reductases
• enantioselectivity
• biocatalysis
• catalysis
• protein