Recurrent Pregnancy Loss in Two Patients with High Density of NK Cells CD56 and CD16 in Ovulatory Cervical Mucus and in Endometrium (Case Report)
Abstract
We describe two patients who suffered from multiple repeated pregnancy losses and with normal hormonal status, humoral autoimmune responses to the panel of phospholipids, and normal karyotyping, without pathological trombophilia. Patients were without any clinical and laboratory symptoms of vaginitis at the time of ovulatory cervical mucus (OCM) sampling and endometrium study.
Blood NK cells were studied by flow cytometry, uterine NK cells CD56+, CD16+ and NK cells in OCM were identified by immunocytochemistry, aPls by ELISA, indirect immunofluorescent method for detection of serum and OCM IgM, IgG antibodies against HHV-6 levels at the time of ovulation. All findings were normal except to high density of NK cells in OCM and in endometrium.
Intramuscular immunoglobulins in special scheme was used for the treatment. Both patients became successfully pregnant, and delivered healthy babies.