Publication at First Faculty of Medicine |
2019
Abstract
In summary, we have identified another factor that can affect the outcome of luciferase reporter assays of sequence variants mapping to gene regulatory regions. We recommend routine assessment of plasmid topology and the use of only plasmids that are supercoiled for transfection.
Good laboratory practice would further suggest that all reporter plasmids to be assayed are prepared simultaneously, that test and control plasmids are analyzed on the same plate, and that a minimum of three technical and three biological replicates are performed for each experiment.