Reactive modifications of DNA nucleobases for labelling, bioconjugations, and cross-linking

Abstract

Modification of DNA with reactive groups and their post-synthetic transformations are useful for labelling, imaging, bioconjugations and cross-linking with other (bio)molecules. This review summarizes the recent progress in this field and covers transformations of oxo groups, cycloadditions, conjugate additions, alkylations, cross-couplings and other reactions.

Examples of applications are given and the practicability and scope of the reactions are discussed.

Keywords

• bacterial RNA polymerase
• nucleic acid
• selective detection
• linked nucleotides
• major groove
• click
• nucleoside
• azide
• oligonucleotides
• peptides