Determination of different kinds of pharmaceuticals in blood plasma by capillary electrophoresis and acetonitrile-based sample stacking
Abstract
Four completely new electrophoretic methods were developed for the sensitive determination of commonly used pharmaceuticals in human or rat plasma. All determinations are coupled with the novel kind of acetonitrile based sample stacking.
All methods are characterised by a low limit of detection and simple sample pretreatment: i) the antiepileptic drug perampanel is determined in the background electrolyte 50 mM chloroacetic acid (pH 2.15), LOD 0.008 µM; ii) the antibiotic ceftazimide is determined in 50 mM chloroacetic acid dissolved in 20% MeOH (pH 2.32), LOD 0.08 µM; iii) the antimicrobial agent pentamidine is determined in 100 mM acetic acid/Tris (pH 4.7), LOD 0.03 µM; iv) the antidiabetic drug metformin is determined in 2.0 M acetic acid (pH 2.15), LOD 0.03 µM.