Electrochemical Enzymatic Biosensors for Determination of Catecholamines in Flow Systems

Abstrakt

Several biosensors (BS) based on flow enzymatic mini-reactors containing a mesoporous silica powder covered by enzyme laccase or tyrosinase were used for determination of L-DOPA, dopamine, noradrenaline, and adrenaline. The silica powder of MCM-41 with covalently bonded laccase was found to be the best reactor filling for the detection of catecholamines.

Relative current response of such BS was 100 % for dopamine, 32.1 % for L-DOPA, 26.2 % for noradrenaline, and 0.71 % for adrenaline. The practical applicability of Lac-MCM41 biosensor was successfully verified by the determination of dopamine and noradrenaline in medical solutions for infusions.

Klíčová slova

• enzymatic biosensor
• catecholamines
• flow analysis
• amperometry
• silver solid amalgam
• tubular detector