Publication at Faculty of Science |
2019
Abstract
Electrochemical behaviour of cholesterol and its precursor 7-dehydrocholesterol was studied using cyclic voltammetry on borondoped diamond and glassy carbon electrode. Inorganic acids (i.e., HClO , H SO 4 2 4, HNO3, and H3PO4) were used for activation of sterol structure by dehydration reaction, similarly to Liebermann-Burchard reaction of cholesterol.
Oxidation potentials of cholesterol and 7-dehydrocholesterol are circa +1.5 V and circa +0.8 V (vs. Ag/AgNO3 in acetonitrile) in optimized supporting electrolyte containing 0.1 mol L-1 perchloric acid in acetonitrile.
The approach is promising for simultaneous detection of both sterols using voltammetry in various physiological (serum) and food matrices.