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Electroanalysis of cholesterol and its precursor 7-dehydrocholesterol on carbon-based electrodes

Publication at Faculty of Science |
2019

Abstract

Electrochemical behaviour of cholesterol and its precursor 7-dehydrocholesterol was studied using cyclic voltammetry on borondoped diamond and glassy carbon electrode. Inorganic acids (i.e., HClO , H SO 4 2 4, HNO3, and H3PO4) were used for activation of sterol structure by dehydration reaction, similarly to Liebermann-Burchard reaction of cholesterol.

Oxidation potentials of cholesterol and 7-dehydrocholesterol are circa +1.5 V and circa +0.8 V (vs. Ag/AgNO3 in acetonitrile) in optimized supporting electrolyte containing 0.1 mol L-1 perchloric acid in acetonitrile.

The approach is promising for simultaneous detection of both sterols using voltammetry in various physiological (serum) and food matrices.