Aldrithiol-2 as a new derivatization agent for double bond localization in unsaturated compounds by mass spectrometry
2019
Abstract
A new method for localizing double bonds in lipids using derivatization with 2,2'-dipyridyl disulfide (Aldrithiol-2) and electrospray ionization mass spectrometry (ESI-MS) is presented. The derivatization introduces a permanent charge to the molecules.
When collisionally activated, the derivatives provide diagnostic peaks useful for establishing the position of the original double bond. Since the permanent charge of the derivative significantly increases the detectability of analytes, the method works well also for lipids of very low polarity like hydrocarbons.