Methodological and diagnostic relevance of IgEs to recombinant allergens Api m 1 and Ves v 5 determined by the multiplex test ImmunoCAP ISAC
Abstract
More than 10% of our ISAC-tested subjects were positive for rApi m1 and rVes v5; of 93 subjects with detailed data, almost one-third had a history of allergy to Hymenoptera stings, and importantly, one third of those subjects had a systemic reaction. Thus, approximately 0,3% up to 1% of ISAC-tested subjects had systemic sting reactions, which is comparable to epidemiology data in population-based studies.
Taken together, if the tested subjects have positive results for rApi m1 and rVes v5 measured on the ISAC system, those results are methodologically highly comparable with the standard CAP uniplex results. Furthermore, since rApi m1 and rVes v5 are the major allergens to honeybee and yellow jacket that cause genuine sensitization and are cross-reactive carbohydrate determinant (CCD)-free, these results could be clinically relevant.Thus, every patient, regardless of the clinical indication, needs to have their positive results precisely interpreted regarding the Hymenoptera allergy.