Monolithic Poly(styrene-co-divinylbenzene) Columns for Supercritical Fluid Chromatography-Mass Spectrometry Analysis of Polypeptide
Abstract
The organic polymer-based monolithic columns have been evaluated as the separation media for analysis of peptides using supercritical fluid chromatography-mass spectrometry (SFC-MS). We demonstrate for the first time the SFC-MS separation of a mixture of polypeptides carried out using poly(styrene-co-divinylbenzene) monolithic columns and carbon dioxide/methanol mobile phase.
A gradient from 2 to 40% methanol modifier containing 0.1% TFA as an acidic additive was applied for the optimized elution and the separation was achieved in less than 3 min. Selected ion monitoring enabled detection of selected masses characteristic of three ionophoric pentadecapeptide antibiotics gramicidin A, B, and C and their two corresponding isoforms.
Furthermore, their identity was confirmed through determination of their [M + 2H](2+), [M + 2Na](2+), and [M + H + Na](2+) ions acquired by positive-ion electrospray ionization-mass spectrometry (ESI-MS).