Novel hybrid electrochemical DNA biosensor for monitoring oxidative DNA damage via oxidation/reduction signals of low molecular weight double-stranded DNA
Abstrakt
Deoxyribonucleic acid (DNA) represents a major target molecule for many damaging agents causing unfavorable changes in a structure of DNA molecule that bind and interact with DNA. Thus, a high demand for reliable tools regarding a better comprehension of the nature of DNA damaging processes still represents one of the main goals in this area.
Herein, we describe a development of a novel hybrid electrochemical DNA biosensor based on an "edge-plane" pyrolytic graphite electrode (EPPGE) in connection with an elementary optimization process providing a closer resolution of the redox processes of low molecular weight double-stranded DNA (dsDNA) at the EPPGE. Subsequent analytical application incorporating an employment of the model structure K [IrCl ] (representative 2 6 of transition metal complexes), and evaluation of its damaging effect in relation to DNA by means of linear sweep voltammetry resp. square-wave voltammetry are also presented.