Abstract
Diagnosis of type I allergy is based on the patient history, provocation testing, and in vitro diagnostic tests. Currently, molecular allergy diagnostics are using in clinical routine, and more than 100 allergenic molecules and extracts are available for in vitro specific immunoglobulin E testing, which can be performed on singleplex or multiplex measurement platforms.
Our claim was to compare the multiplex ImmunoCAP ISAC (Thermo Fisher Scientific, Sweden) and the multiplex ALEX (Macro Array Diagnostics, Austria) platform. A good correlation of presently used methods to detect serum sIgE was observed.
Multiplex testing of allergen-specific IgE is one of the methods for detection allergy type I in patients. It helps clinicians with diagnosing and establishing treatment for these patients.