Gene expression of cytokinesis regulators PRC1, KIF14 and CIT has no prognostic role in colorectal and pancreatic cancer
Abstrakt
Colorectal cancer is one of the most frequent cancers and pancreatic adenocarcinoma is among the most fatal and difficult to treat. New prognostic biomarkers are urgently needed to advance the treatment of these malignancies.
PRC1, KIF14 and CIT play an important role in cytokinesis, strongly connected with cancer progression, and have prognostic potential. We investigated prognostic relevance of these genes for colorectal and pancreatic cancer.
We followed gene expression of PRC1, KIF14 and CIT by qPCR in colorectal carcinomas and paired distant unaffected mucosa from 67 patients and tumors and paired non-neoplastic control tissues from 48 patients with pancreatic adenocarcinoma. The extent of transcript dysregulation between tumor and control tissues and between groups of patients divided by main clinical characteristics was determined.
Finally, we evaluated associations of transcript levels in tumors with disease-free interval and overall survival. PRC1, KIF14 and CIT transcripts were overexpressed in tumors compared to control tissues and strongly correlated together in both patient cohorts after correction for multiple testing.
However, we have not found significant associations of transcript levels in target genes with survival. Taken together, study shows overexpression and mutual correlation of the followed cytokinesis regulators with no clear prognostic value.