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Biological variation of proprotein convertase subtilisin/kexin type 9 (PCSK9) in human serum

Publication at First Faculty of Medicine, Third Faculty of Medicine |
2021

Abstract

BACKGROUND: Proprotein convertase subtilisin/kexin type 9 (PCSK9) is involved in the regulation of LDL receptors. Inhibition of PCSK9 increase uptake of LDL-particles and pathogen-associated molecular patterns (PAMPs).

The aim of our study was to evaluate biological variation of serum PCSK9. METHODS: Within-subject (CV(I)) and between-subject (CV(G)) biological variations were assessed in 14 healthy volunteers in a 6-week protocol (7 samples, equidistant time intervals).

Serum concentration of PCSK9 was measured by a Quantikine ELISA assay (R&D systems, Bio-Techne Ltd.,UK) on a DS2 ELISA reader (Dynex Technologies GmbH, Germany). Precision (CV(A)) was assessed by duplicate measurements.

Two methods with different levels of robustness were used for the estimation of CV(I), SD-ANOVA and CV-ANOVA method. We calculated the index of individuality and reference change values.

The experiment was fully compliant with EFLM database checklist. RESULTS: The within-subject values of PCSK9 in healthy persons, as calculated by two statistical methods, were 23.2% (SD-ANOVA with CV(A) of 5.6%) and 26.6% (CV-ANOVA with CV(A) of 4.8%).

The CV(G) was 10.9% (SD-ANOVA), index of individuality and RCV were 2.13 and 66.3%, respectively. CONCLUSIONS: The high index of individuality indicates that common reference intervals can be used to interpret serum PSCK9 values.