Abstract
The invention provides a module for an on-line permeation test monitoring system, comprising an assembly of two plastic containers, namely a larger main container for culture medium with a marker and/or tested substance, into which a smaller container forming a cell cultivation insert, filled on at least one side of its inner surface by a continuous cell monolayer, is inserted, wherein the smaller container forms a donor compartment, and the interspace between the inserted smaller container and the larger container defines an acceptor compartment, and wherein the main container further comprises a lid for closing the main container, the lid is provided with at least one inlet and outlet channel for the donor compartment and at least one inlet and outlet channel for the acceptor compartment and at the same time the channels being configured to assure the connection of these compartments to an external flow system. Preferably, the plastic module is the 3D-printed module.