Chemoenzymatic one-pot reaction from carboxylic acid to nitrile via oxime

Abstract

We report a new chemoenzymatic cascade starting with aldehyde synthesis by carboxylic acid reductase (CAR) followed by chemical in situ oxime formation. The final step to the nitrile is catalyzed by aldoxime dehydratase (Oxd).

Full conversions of phenylacetic acid and hexanoic acid were achieved in a two-phase mode.

Keywords

• chemoenzymatic cascade
• carboxylic acid reductase
• aldoxime dehydratase
• nitrile
• oxime