The Impact of Fullerenes as Doxorubicin Nano-Transporters on Metallothionein and Superoxide Dismutase Status in MCF-10A Cells
Abstract
This study aimed to synthesise C-60-DOX complexes followed by the analysis of their effect on the concentration of metallothionein (MT) as a non-enzymatic antioxidant and on the concentration and activity of superoxide dismutase (SOD) as an antioxidant enzyme in healthy human mammary MCF-10A cells. Dynamic light scattering and electrophoretic light scattering were used to establish the size and zeta potential of the complexes.
The MT and SOD concentrations were determined using the ELISA method; SOD activity was determined by tetrazolium salt reduction inhibition. Lower MT concentration following exposure of cells to both DOX and C-60 fullerene compared to the control sample was found.
However, the concentration of this protein increased as a consequence of the C-60-DOX complexes action on MCF-10A cells compared to the control. C-60 used alone did not affect the concentration and activity of SOD in MCF-10A cells.
Application of free DOX did not activate cellular antioxidant defence in the form of an increase in SOD concentration or its activity. In contrast treatment of cells with the C-60-DOX complex resulted in a decrease in SOD1 concentration and a significant increase in SOD activity compared to cells treated with free DOX, C-60 and control.
Thus, it was found that C-60-DOX complexes showed potential for protective effects against DOX-induced toxicity to MCF-10A cells.