Dialysis in Microliter Sample Volume Connected via Flow-gating Interface with Rapid Capillary Electrophoresis
2021
Abstract
A new analytical apparatus was described based on on-line connection of the electrophoresis in short capillary with a dialysis unit allowing dialysis from the microliter volume of the sample to the sub-microlitre volume of the acceptor solution. The properties of the apparatus were tested with model solutions of a mixture of inorganic cations (K+, Ba2+ and Na+) and organic molecules (creatinine, histidine and arginine).
The air-assisted flow-gating interface was manufactured in the form of a chip cast from PDMS. Basic properties of the apparatus, namely concentration dependences and separation parameters were presented.