The electrochemical behaviour of two structurally different neurotransmitters, dopamine and serotonin, in pH 7.4 common biomimicking and tissue culture media, namely in phosphate buffered saline, HEPES buffered saline and complex Neurobasal media, was studied using an anodically activated (oxygen-terminated) boron doped diamond electrode. Moreover, the influence of human serum albumin on the anodic potential limits evaluated in buffered physiological solutions and on the voltammetric behaviour of dopamine and serotonin was thoroughly investigated.