Charles Explorer logo
🇨🇿

Biomimetic hierarchical nanofibrous surfaces inspired by superhydrophobic lotus leaf structure for preventing tissue adhesions

Publikace na Lékařská fakulta v Plzni, Matematicko-fyzikální fakulta |
2022

Tento text není v aktuálním jazyce dostupný. Zobrazuje se verze "en".Abstrakt

Undesirable tissue adhesions remain one of the most common and dreaded postoperative complications. Biocompatible nanofibrous mats with antiadhesive surfaces represent a promising barrier method for preventing the formation of adhesions.

The material developed in this work was inspired by the natural superhydrophobic lotus leaf nanostructure, which was mimicked by a unique combination of needleless electrospraying and electrospinning technology of poly-e-caprolactone (PCL). The surface hydrophobicity of electrosprayed nanodroplets was further enhanced by cold plasma modification using the chemical vapor deposition (CVD) method with hexamethyldisiloxane (HMDSO).

The treatment led to a successful decrease in surface wettability of our samples. Morphology (scanning electron microscopy), wettability (contact angle) and chemical composition (FTIR analysis) were observed for a period of six months to track possible changes; the obtained results verified the presence of HMDSO during the whole time period.

Cytocompatibility was confirmed in vitro with 3T3 mouse fibroblasts according to the norm ISO 10993-5. Cell adhesion and proliferation were assessed in vitro by metabolic MTT assay and fluorescence microscopy after 4, 7, and 14 days.

Antiadhesive behaviour was confirmed by atomic force microscopy