Abstract
Pulsed electric fields have already wide use and further great potential for novel applications in biomedicine and food industry. Although not fully explored, one mechanism of the electric pulses action in biological samples is through the electric field induced oxidation.
In this context, our long term aim is to employ endogenous biological chemiluminescence (also termed us ultra-weak photon emission - UPE) for a label-free interrogation of the oxidative effects of us-ns electric pulses in biosamples. In this work we demonstrated the first step - that the UPE indeed reports oxidation rate.
On the yeast cell culture as a model system we showed that UPE is generated both when oxidative substances are added to the sample or when the oxidation is produced during the normal metabolism during the growth of the cell culture.