Dysregulation of butyrylcholinesterase, BCHE gene SNP rs1803274, and pro-inflammatory cytokines in occupational workers
Abstrakt
Background.People in different occupations are exposed to a variety of xenobiotics which affect the health and physio-logical processes of the body. Butyrylcholinesterase (BChE), has been reported to play neuronal and non -neuronal roles, though its exact function is yet to be established.
This study aimed to find the status and role of BChE in seven different occupational groups; gasoline fillers, auto-mechanics, carpenters, textile shop workers, furniture shop workers, electricians, and office workers.Methods.A total of 400 samples were screened. BChE activity was determined by Worek et al. method based on Ellman's principle.
Pro-inflammatory cytokines were determined by ELISA. Genotypic analysis of the K-variant of BCHE gene SNP was carried out by standard molecular methods.
Among seven groups, office workers were taken as a control to compare the results with all other occupational groups.Results.The results revealed a significant decrease in BChE activity in gasoline fillers (79.52%) followed by carpenters (73.49%), auto mechanics (39.76%), textile shop workers (18.07%), electricians (10.84%), and furniture shop workers (7.23%). TNF-alpha, IL-6, and IL1-13 were elevated in all groups.
IL-6 and IL1-13 in gasoline fillers, and electricians were not statistically significantly increased. Binomial regression to determine the odd ratio was found to be significant (p < 0.05) in all groups.
However, correlation (Pearson) did not reveal significance be-tween different biochemical parameters. Genotypic analysis of the K-variant SNP of the BCHE gene showed a significant association with occupational groups when compared with control which indicates a possible asso-ciation with xenobiotics exposure and the physiological role of K-variant in understudied occupational groups.Conclusion.The study concluded that BChE and its gene SNP rs 1803274 and proinflammatory cytokines significantly dysregulates under the exposure to cumulative multiple xenobiotics in different occupational groups which may lead to pathophysiological conditions.