Oxidative stress and DNA fragmentation in frozen/thawed common Carp Cyprinus carpio sperm with and without supplemental proteins
Abstract
Using cryopreservation techniques can increase the effectiveness of reproducing cultured fish species by ensuring a dependable supply of sperm, although the quality of the sperm could be impacted by the procedures involved. The goal of this study was to investigate the effect of pu-rified seminal plasma transferrin (Tf), bovine serum albumin (BSA), and antifreeze protein (AFP) types I and III at 1 µg mL(-1) on relevant characteristics of cryopreserved sperm from common carp Cyprinus carpio.
We compared oxidative stress indices, antioxidant activity, and DNA fragmen-tation of fresh sperm to that frozen with extender only or with Tf, BSA, or AFP types I and III. Fresh sperm had significantly lower levels of thiobarbituric acid reactive substances (TBARS) compared to samples that underwent cryopreservation without protein treatment, which resulted in 0.54 +/- 0.06 nmol/10(8) cells of TBARS.
Carbonyl derivatives of proteins (CP) decreased significantly (ANOVA; P > 0.05) in carp sperm with addition of Tf, AFPI, and AFPIII. Significant differences in superoxide dismutase (SOD), glutathione reductase (GR), and glutathione peroxi-dase (GPx) activity were seen in sperm supplemented with Tf, BSA, AFPI, and AFPIII from those without.
Significantly less DNA damage, expressed as percent tail DNA (11.56 +/- 1.34) and olive tail moment (0.59 +/- 0.13), was recorded in samples cryopreserved with Tf. The findings indi-cated that addition of Tf, BSA, AFPI, or AFPIII to cryopreservation medium is beneficial to sperm preservation.
The mechanisms through which these proteins act positively on sperm need to be further investigated.