Abstract
In the past, blood coatings were often used not only for aesthetics but also as a functional material, which, however, was neglected in many written sources as a consequence of their mundaneness. The clarity of the location and historical objects blood coatings are on, is hindered due to the absence of historical knowledge in this field.
For these reasons, their analysis is crucial. The goal of this work was to analyse the protein composition of 24 model blood coatings on wood using nano-Liquid Chromatography - Electrospray Ionization - Quadrupole - Time of Flight Mass Spectrometry (nano-LC-ESI-Q-TOF MS).
The results were compared with a worldwide protein database of plant and animal proteins (UniProt), and the obtained particular proteins, were compared to the original substances from which the blood coatings were prepared. Using the same procedure, a sample from historical object at Horní Bečva from 18th century was analysed.