prezentace v rámci WDS: MICROMANIPULATION AS A NEW INSTRUMENT FOR RESEARCH OF BIOMEMBRANE STRUCTURE AND ITS MECHANICAL PROPERTIES
Abstract
In cells, various membrane events are influenced and sometimes even controlled by changing of the tension on the membrane or their curvature (for example movement of the cells, influence on membrane proteins etc.). Micromanipulation technique brings new, so far not fully explored possibilities to this biomembrane research, as it enables direct modifications of membrane mechanical properties of giant unilameral vesicules (GUVs), simulating cell membrane.
The combination of micromanipulation with other methods such as fluorescence microscopy will allow us to open the door to other exciting experiments we plan to perform in later stages of this project. Especially we are interested in the combination of micromanipulations with our frequently used method MC-FRET, which allows us to detect and characterize membrane nanodomains. Also studying of the peptide-membrane interactions and influence of the peptides on mechanical properties of the cells is planned in near future.
My lecture will however focus mainly on the basics of micromanipulation as the new method, which we are currently introducing here at Heyrovsky Institute in Fluorescence group. Also some future outlook will be provided, as well as first obtained results.
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