The differences in the count of B lymphocytes in atopic dermatitis patients with and without dupilumab therapy and in healthy subjects in pollen season and out of pollen season
Abstract
The aim of the study is to measure the absolute count of leukocytes, lymphocytes, absolute B lymphocytes, relative count of B lymphocytes with their subsets and the count of DC5+, CD22+ and CD73+ B lymphocytes in atopic dermatitis patients in pollen season and out of pollen season nad compare in with these counts in healthy subjects. We examined two grups of AD patients; patients withoutdupilumab treatment (21 patients - 6 men, 15 women, age 36,2 years) and patients treated with dupilumab (13 patients - 7 men, 6 women, age 43,4 yers).
Laboratory test to determine the parameters of interest were performed in these patients during the pollen season (June-August) and outside the pollenseason (October-February). In pollen season, the count of toptal memory B lymphocytes is significantly lower the countof naive B lymphocytes is significantly higher in both controls and AD patients compared to the cont out of pollen season.
Out of pollen season we the lower count of relative CD22 B lymphocytes in both groups of AD patients compared controls. Significant changes occur also in healthy subjeckts in pollen season in the count of memory, naive and non switched B lymphocytes.