Abstract
Topical chemotherapy approaches are relevant for certain skin cancer treatments. This study observes that cabazitaxel (CTX), a broad-spectrum second-generation taxane cytotoxic agent, can be dissolved in alpha-tocopherol at high concentrations exceeding 100 mg mL(-1). 2D nuclear magnetic resonance (NMR) analysis and molecular dynamics (MD) are used to study this phenomenon.
The addition of 30% dimethyl sulfoxide (DMSO) to the alpha-tocopherol/CTX solution improves its working viscosity and enhances CTX permeation through human skin in vitro (over 5 mu g cm(-2) within 24 h), while no detectable drug permeates when CTX is dissolved in alpha-tocopherol alone. In a transepidermal water loss assay, the barrier impairment induced by CTX in 30% DMSO in alpha-tocopherol, but not in pure DMSO, is reversible 8 h after the formulation removal from the skin surface.
Antitumor efficacy of the topical CTX formulation is evaluated in nude mice bearing A431 human squamous carcinoma skin cancer xenografts. With topical application of concentrated CTX solutions (75 mg mL(-1)), tumor growth is significantly suppressed compared to lower concentration groups (0, 25, or 50 mg mL(-1) CTX).
Taken together, these findings show that topical delivery of CTX using a DMSO and alpha-tocopherol solvent warrants further study as a treatment for skin malignancies.