A Lotus japonicus nodulation system based on heterologous expression of the fucosyl transferase NodZ and the acetyl transferase NolL in Rhizobium leguminosarum
2000
Abstract
Heterologous expression of NodZ and NoIL proteins in Rhizobium leguminosarum by. viciae led to the production of acetyl fucosylated lipo-chitin oligosaccharides (LCOs), indicating that the NoIL protein obtained from Mesorhizobium loti functions as an acetyl transferase. We show that the NoIL-dependent acetylation is specific for the fucosyl penta-N-acetylglucosamine species.
In addition, the NoIL protein caused elevated production of LCOs, Efficient nodulation of Lotus japonicus by the NodZ/NoIL-producing strain was demonstrated. Nodulation efficiency was further improved by the addition of the ethylene inhibitor L-alpha-(2-aminoethoxyvinyl) glycine (AVG).