This study aimsto address sperm reproductive parameters and testis germ cell pathologies related to testicular germ cell tumour (TGCT) from both the clinical and experimental angle. The goal is to deliver novel diagnostic strategies for the evaluation of sperm samples collected from TGCT men prior to surgery and after the therapeutic intervention, in order to monitor and compare the quality of fertility parameters and recommend the first choice of cryopreserved semen sample for assisted reproduction.