Abstract
The use of acridine derivatives bearing a substituent bound to the aromatic core via a carboxamide group containing a carbon chain terminated by an azido group serving for azido-alkyne cycloaddition as intermediates for the preparation of intercalators increasing the binding strength of complementary DNA chains and affecting its melting temperature. This substituent is bound at any free position of the tricyclic core, the core modification being used to fix the bond to the DNA double helix by hydrogen bonding with the nitrogen and oxygen of the guanine, and further by hydrogen bonding of the amide hydrogen via a water molecule to the phosphate group between the corresponding guanosine and the adjacent nucleoside.