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Characterization of the microgel particles by the light microscopy and cryo-SEM

Publication at Central Library of Charles University |
2023

Abstract

Polymer hydrogels and/or microgels can be studied by several microscopic techniques. Light-microscopy (LM) visualizes samples in their natural state - submerged in water, but suffers from lower resolution.

Cryo-TEM microscopy exhibits the best resolution, but the method is not suitable for particle with sizes >300 nm. Cryo-SEM offers sufficient resolution and, at the same time, it allows observing even large particles.