The aim of the empirical questionnaire study of all Emergency Medical Service's in the Czech Republic was to identify the amount of burnout syndrome symptoms, firstly (2003) without any previous intervention techniques, and secondly (2005) after preventive and intervention strategies used in some EMS organisations. Statistically significant influences of the length of practice in EMS was found, and the profession of dispatcher had significantly higher score of burnout symptoms in comparison with all other professions in EMS.