Publication at First Faculty of Medicine |
2004
Abstract
Objective:The aim of this study was to define specific genetic alterations which are common in bone metastases in renal cancer patients. Methods: Tumor DNA from 31 metastases and 13 related primary tumors was extracted from paraffin embedded tissue sections.
DOP-PCR was performed to amplify the whole DNA. After labelling by PCR, CGH was performed according to standard protocols.
Conclusions: Bone metastases from renal cell carcinoma are characterized by typical genetic alterations. Changes leading to metastasizing happen early in tumor pathogenesis.
However, further accumulation of genetic changes occurs in metastases leading to a more complex genetic pattern which might be necessary for progression to clinically relevant metastases.