A Capillary GC Method Using Nitrogen Phosphorus Detection for Determination of Topiramate in Patients with Epilepsy
2007
Abstract
A new capillary gas chromatographic assay with nitrogen phosphorus detection for the determination of antiepileptic drug topiramate in the serum of epileptic patients was developed and validated. The sample procedure included a liquid-liquid extraction of 0.1 ml of alkalized sample with ethyl acetate.
The organic solvent was evaporated, and the injection on column and flash methylation of topiramate and internal standard was performed