Publication at Faculty of Science |
2010
Abstract
Separation systems with a zirconia-based polystyrene HPLC column were characterized by different approaches, which allowed the recognition of interactions participating in the separation environments. General chromatographic tests by Walters, Engelhardt and Galushko were applied to evaluate the fundamental properties of the systems – hydrophobicity and polarity.
The complex model of linear free energy relationship described the interactions from the qualitative and quantitative points of view more in detail. Application of a set of basic compounds revealed the contribution of ion-exchange interactions participating in the separation systems.