Ultra-high-performance liquid chromatography fingerprinting method for chemical screening of metabolites in cultivation broth

Abstract

A fingerprinting method for chemical screening of microbial metabolites, potential antibiotics, in spent cultivation broths is described. The method is based on high-throughput ultra-high-performance liquid chromatography (UHPLC) separation with UV detection (photodiode array detector).

Keywords

• UHPLC
• High-throughput fingerprinting
• Secondary metabolites
• Antibiotics
• Cultivation broth
• Actinomycetes